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高保真 DNA 聚合酶 Z1（HiFi DNA Polymerase Z1）介绍

一、产品概述

高保真 DNA 聚合酶 Z1（HiFi DNA Polymerase Z1）是一款专为精准 DNA 复制设计的酶制剂，通过3'→5' 外切酶活性（校对功能）实现低至10⁻⁶级错配率，显著优于普通 Taq 酶（10⁻⁵级）。其核心优势在于长片段扩增能力（可扩增长达 38 kb 的 DNA）和复杂模板兼容性（如 GC-rich 或重复序列），适用于对保真性和扩增效率要求极高的分子生物学实验。
技术特性：
  • 高保真性：通过内置校对机制，确保每轮 PCR 的错误率低于 0.01%，尤其适合基因克隆、突变检测和测序模板制备。
  • 快速延伸：延伸速率达1 kb / 秒，支持高效长片段扩增（如人类基因组 22 kb 片段）。
  • 热稳定性：在 95℃下的半衰期超过 2 小时，兼容高温循环条件。
  • 抑制剂耐受性：可在含腐殖酸、血液或粪便等复杂样本中稳定工作。

二、产品规格与类型

  1. 纯度级别：
    • 分子生物学级：OD₂₆₀/OD₂₈₀ ≥1.8，无 DNase/RNase 污染，适用于 CRISPR 编辑、二代测序（NGS）等敏感应用。
    • 工业级：含微量添加剂，适合大规模生产（如疫苗开发、工业酶工程）。
  1. 包装形式：
    • 溶液型：2 U/μL（Tris-HCl 缓冲液，pH 8.5），包装规格从 250 U 到 10,000 U 不等。
    • 冻干型：粉末形式，需用户自行复溶，适合长期保存（-20℃保质期 3 年）。
  1. 配套试剂：
    • 专用缓冲液：含 Mg²⁺和 dNTP，优化 GC-rich 模板扩增。
    • 增强剂：如甜菜碱或 DMSO，提升复杂模板的扩增效率。

三、核心应用领域

  1. 分子生物学研究：
    • 长片段 PCR：支持从基因组 DNA 中扩增超过 30 kb 的片段（如病毒全基因组、线粒体 DNA）。
    • 定点突变：通过高保真复制减少引入额外突变的风险。
    • 基因克隆：直接克隆 PCR 产物至表达载体，避免 TA 克隆的加尾步骤。
  1. 合成生物学与基因工程：
    • DNA 组装：支持 Gibson Assembly 等无缝克隆技术，实现多片段拼接（如 24.4 kb 连续片段）。
    • 密码子优化：精确合成含稀有密码子的基因，提升异源表达效率。
  1. 工业与诊断：
    • 二代测序（NGS）：用于文库制备，确保均一扩增和低序列偏差（如 cfDNA 建库）。
    • 快速检测：结合等温扩增技术（如 RPA），实现病原体的高灵敏检测。

四、注意事项

  • 酶活性干扰：避免与 EDTA 或 SDS 等强螯合剂直接接触。
  • 保存条件：溶液型需 - 20℃避光保存，避免反复冻融；冻干型可在 - 80℃长期保存。
  • 安全防护：操作时戴手套，避免吸入粉末或接触皮肤。


High-Fidelity DNA Polymerase Z1 (HiFi DNA Polymerase Z1) Product Introduction

1. Product Overview

HiFi DNA Polymerase Z1 is a precision-engineered enzyme designed for accurate DNA replication, achieving a mutation rate as low as 10⁻⁶ through its built-in 3'→5' exonuclease activity (proofreading function), significantly outperforming conventional Taq enzymes (10⁻⁵) . Its core strengths lie in long-fragment amplification (up to 38 kb) and compatibility with complex templates (e.g., GC-rich or repetitive sequences), making it ideal for molecular biology experiments requiring high fidelity and efficiency.
Technical Features:
  • Ultra-High Fidelity: Ensures <0.01% error rate per PCR cycle, critical for gene cloning, mutation detection, and sequencing template preparation.
  • Rapid Extension: Extends at 1 kb/second, enabling efficient amplification of long fragments (e.g., 22 kb human genomic DNA) .
  • Thermostability: Maintains activity for >2 hours at 95°C, suitable for high-temperature cycling.
  • Inhibitor Tolerance: Works efficiently in complex samples containing humic acid, blood, or feces.

2. Product Specifications

  1. Purity Grades:
    • Molecular Biology Grade: OD₂₆₀/OD₂₈₀ ≥1.8, DNase/RNase-free, suitable for CRISPR editing and next-generation sequencing (NGS).
    • Industrial Grade: Contains minor additives for large-scale production (e.g., vaccine development, industrial enzyme engineering).
  1. Formats:
    • Liquid: 2 U/μL in Tris-HCl buffer (pH 8.5), available in 250 U to 10,000 U packaging.
    • Lyophilized: Powder form for long-term storage (-20°C shelf life: 3 years).
  1. 配套试剂:
    • Optimized Buffer: Contains Mg²⁺ and dNTPs for GC-rich template amplification.
    • Enhancers: Betaine or DMSO to improve complex template performance.

3. Core Applications

  1. Molecular Biology Research:
    • Long-Fragment PCR: Amplifies >30 kb genomic DNA (e.g., viral genomes, mitochondrial DNA) .
    • Site-Directed Mutagenesis: Minimizes off-target mutations during plasmid editing.
    • Cloning: Directly clones PCR products into expression vectors without TA cloning.
  1. Synthetic Biology & Genetic Engineering:
    • DNA Assembly: Enables seamless cloning (e.g., Gibson Assembly) for multi-fragment 拼接 (e.g., 24.4 kb contiguous fragments) .
    • Codon Optimization: Synthesizes genes with rare codons for heterologous expression.
  1. Industrial & Diagnostic Applications:
    • NGS Library Preparation: Generates high-quality libraries from 1 ng cfDNA with uniform coverage .
    • Rapid Detection: Combines with isothermal amplification (e.g., RPA) for sensitive pathogen detection.

4. Notes

  • Enzyme Interference: Avoid direct contact with EDTA or SDS.
  • Storage: Store liquid form at -20°C (protect from light); lyophilized form at -80°C.
  • Safety: Wear gloves to prevent inhalation or skin contact.

7. Application Cases

  1. Cancer Early Detection:
    • Amplifies tumor mutations from cfDNA for NGS-based screening (sensitivity: 95.42%) .
  1. Synthetic Biology:
    • Uses STI PCR to amplify 38 kb DNA fragments for artificial genome assembly .
  1. Industrial Enzyme Engineering:
    • Clones and optimizes industrial enzyme genes (e.g., cellulases) for enhanced thermostability.

For detailed technical specifications or ordering information, please contact suppliers directly or visit their official websites.


特别提醒：

1. 试剂使用干冰填充运输，收到货后及时放入-80℃储存。

2. 避免反复冻融，否则会对试剂活性造成一定影响。